Study examines effects of calycosin on progression of breast cancer cells

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Aging-US published “Calycosin inhibits migration and invasion of breast cancer cells by suppressing EMT via BATF / TGF-β1” which reported that in this study the authors investigated the effects of calycosin on progression breast cancer cells and their underlying mechanisms. Calycosin dose- and time-wise inhibited the proliferation, migration and invasion by T47D and MCF-7 breast cancer cells by down-regulating the expression of the basic transcription factor of leucine zipper.

In addition, BATF promoted the migration and invasiveness of breast cancer cells by increasing the levels of mRNA and TGFβ1 proteins.

Bioinformatics analysis, luciferase double reporter assays and chromatin immunoprecipitation assays confirmed the presence of BATF binding sites in the promoter sequence of the TGFβ1 gene.

Calycosin treatment inhibited the epithelial-to-mesenchymal transition of breast cancer cells by significantly increasing the levels of E-cadherin and decreasing the levels of N-cadherin, Vimentin, CD147, MMP-2 and MMP- 9 by downregulation of BATF and TGFβ1. TGFβ1 knockdown reduced migration and invasiveness of BATF-overexpressing breast cancer cells, while incubation with TGFβ1 enhanced migration and invasiveness of calycosin-treated breast cancer cells.

These results, published in Aging-US, demonstrated that calycosin inhibited EMT and breast cancer cell progression by suppressing BATF / TGFβ1 signaling. This suggests that calycosin would be a promising treatment option for patients with breast cancer.

“Breast cancer is the most common cause of cancer-related death in women around the world.”

Yuzhong Zheng and Dr Fenglian Yang, Hanshan Normal University

Improvements in diagnosis and treatment strategies have increased survival rates in patients with breast cancer over the past decades, but the prognosis for patients with advanced breast cancer remains bleak due to high rates of drug resistance and disease recurrence.

Phytoestrogens are non-steroidal compounds of plant origin which are structurally similar to 17β-estradiol and which demonstrate estrogenic effects on breast cancer cells.

Calycosin inhibited the in vitro growth of pancreatic cancer cells by inducing cell cycle arrest and apoptosis; however, it also induced metastatic progression of pancreatic cancer in an orthotopic tumor xenograft mouse model by modulating the tumor microenvironment.

However, the functional role of BATFs in breast cancer is poorly understood.

Therefore, in this study, these authors investigated the effects of calycosin on the progression of breast cancer cells and the underlying mechanisms, including its effect on BATF expression and functions in breast cancer cells. .

The Zheng / Yang research team concluded in their US aging research result: “Our results demonstrated that calycosin inhibited the progression of breast cancer cells by suppressing EMT via BATF / TGFβ1. Therefore, calycosin is a promising candidate for the treatment of breast cancer. “

Source:

Journal reference:

Zhang, Z., et al. (2021) Calycosin inhibits migration and invasion of breast cancer cells by suppressing EMT via BATF / TGF-β1. doi.org/10.18632/aging.203093.


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